Glucoamylase CAS 9032-08-0
CELLULASE CAS 174063-87-7
27 8 月, 2020Glucoamylase Powermix 2.0
27 8 月, 2020
Glucoamylase Product Characteristics
| Declared Activity | 150,000 u/ml |
| Production Organism | Aspergillus niger |
| Physical Form | Liquid |
| Color | Brown Color may vary from batch to batch. Color intensity is not an indication of enzyme activity. |
| Odour | Normal microbial fermentation odour |
| Production Method | Produced by submerged fermentation, separation and extraction technology. |
| Definition of Unit | 1 unit of Glucoamylase equals to the amount of enzyme which hydrolyzes soluble starch to get 1mg glucose at 40℃ and pH4.6 in 1h. |
Glucoamylase Product Specification
| Lower Limit | Upper Limit | |
| Enzyme activity | 150,000 u/ml | |
| Lead | 5 mg/kg | |
| Arsenic | 3 mg/kg | |
| Total viable count | 50,000 CFU/ml | |
| Coliform Bacteria | 30 CFU/ml | |
| Escherichia coli | 10 CFU/ml 3 MPN/ml |
|
| Salmonella | Not Detected/25ml |
Glucoamylase Reaction
This enzyme mainly comes from Rhizopus and Aspergillus niger. In the production, the mutant strain AS.3.4309 (abbreviated as UV-11) of Aspergillus niger is mainly used as the production bacteria, with corn flour (10%-12%), soybean meal 4%, corn syrup 2%, bran 1%, etc. Culture medium, deep culture for 100h; fermentation broth is refined and dried to obtain powder.
It is isolated from the culture medium after controlled fermentation of Aspergillus niger var. Uses: Preparation of starch syrup and fruit juice; manufacture of cheese.
Glucoamylase Reaction Parameters
| Activity Temperature | 30℃-65℃ |
| Optimum Temperature | 55℃-60℃ |
| Activity pH | 3.5-6.0 |
| Optimum pH | 4.0-5.0 |
Benefits
• Maximizes conversion of starch substrates to fermentable sugars
• Produces highly attenuated beers in a cost-effective manner
• Maintain consistent fermentability, regardless of varying raw material qualities
Dosage
The recommended dosage is 0.4-1.0L of the enzyme preparation per ton of total raw materials, added at mashing-in. However, the optimal dosage depends on the specific process parameters and should be determined by testing different dosages at the brewery.
Glucoamylase Recommended Uses
This product can catalyze the hydrolysis of starch to produce beer, rice wine, sauce, monosodium glutamate and antibiotics; it can also be used in the production of glucose, caramel and dextrin. Our country stipulates that it can be used in the production of fermented wine, distilled wine, alcohol, and starch syrup, and should be used in appropriate amounts according to production needs.
Used for glycogen determination in yeast cells; synthesis of iso-oligosaccharides by glucoamylase
Biochemical research
Glucoamylase Recommended application
Glucoamylase is a kind of amylase, which can be used for the saccharification of starch materials in alcohol and wine, amylase and other fermentation industries.
Glucoamylase Recommended Chemical nature
Near white to light brown amorphous powder, or light brown to dark brown liquid, can be dispersed in food grade diluent or carrier, and can also contain stabilizers and preservatives. The α-1,4- and α-1,6-glycoside bonds of polysaccharides (starch, glycogen, etc.) can be hydrolyzed into glucose. It is soluble in water, almost insoluble in ethanol, chloroform and ether.
Glucoamylase Recommended Storage
| Best before | When stored as recommended, the product is best used within 12 months from date of delivery. |
| Storage at customer’s warehouse | 0-15℃ |
| Storage Conditions | This product should be stored in a cool and dry place in sealed container, avoiding insolation, high temperature and damp. The product has been formulated for optimal stability. Extended storage or adverse conditions such as higher temperature or higher humidity may lead to a higher dosage requirement. |
Glucoamylase Packaging
25kgs/drum; 1,125kgs/drum.
Safety and Handling Precautions
Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.